کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2048002 1074050 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A non-coding transcript of nephronectin promotes osteoblast differentiation by modulating microRNA functions
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
A non-coding transcript of nephronectin promotes osteoblast differentiation by modulating microRNA functions
چکیده انگلیسی

We investigated the roles of the non-coding transcripts and found that expression of a fragment containing the 3′-untranslated region (3′-UTR) of nephronectin in osteoblast progenitor cells MC3T3-E1 promoted cell differentiation dramatically. We hypothesized that the ectopically expressed 3′-UTR binds microRNAs and modulates their functions. β-Catenin and GSK3β were up-regulated in the 3′-UTR-transfected cells, contributing to the increased cell differentiation, through reduction of EGFR and ERK phosphorylation. Activator of GSK3β promoted differentiation, while inhibitor of GSK3β blocked differentiation. Our results indicate that the non-coding transcripts are important in regulating cell activities and may have potential application for modulating endogenous microRNA functions.


► Expression of nephronectin 3′-untranslated region (3′-UTR) promotes osteoblast differentiation.
► The 3′-UTR binds endogenous microRNAs and modulates their functions.
► β-Catenin and GSK3β and the associated signal pathway are up-regulated by the 3′-UTR.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 585, Issue 16, 19 August 2011, Pages 2610–2616
نویسندگان
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