کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2048014 | 1074053 | 2012 | 6 صفحه PDF | دانلود رایگان |
Lactoferrin (Lf) is known to have anti-inflammatory activity, but the mechanisms of action by Lf remain to be elucidated. Here, we demonstrated that TNF-α-induced expression of intercellular adhesion molecule-1 (ICAM-1) was down-regulated by Lf in a DNA-binding dependent manner at transcriptional level in endothelial cells. Our results showed that Lf bound to a DNA region in the ICAM-1 promoter in vitro as well as in chromatin context. Lf inhibited binding of NF-κB to a proximal NF-κB site in ICAM-1 promoter. This type of repression represents an additional mechanism for the action of Lf in regulation of gene expression.
► Lactoferrin (Lf) inhibits TNF-α-stimulated ICAM-1 expression in endothelial cells.
► Lf down-regulates TNF-α-stimulated ICAM-1 expression at the transcriptional level.
► Lf binds to the proximal NF-κB site in human ICAM-1 promoter in vitro and in vivo.
► Lf interferes with NF-κB for binding to the ICAM-1 promoter region.
Journal: FEBS Letters - Volume 586, Issue 3, 3 February 2012, Pages 229–234