کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2048056 | 1074056 | 2013 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Transient receptor potential vanilloid-2 mediates the effects of transient heat shock on endocytosis of human monocyte-derived dendritic cells Transient receptor potential vanilloid-2 mediates the effects of transient heat shock on endocytosis of human monocyte-derived dendritic cells](/preview/png/2048056.png)
• Short heat shock decreased the endocytotic activity of human dendritic cells (DCs).
• Heat shock-induced robust membrane currents in DCs.
• These effects were mediated by TRPV2 ion channels.
Our goal was to investigate the effect of heat shock on human monocyte-derived dendritic cells (DCs) and to dissect the role of thermosensitive transient receptor potential (TRP) channels in the process. We provide evidence that a short heat shock challenge (43 °C) decreased the endocytotic activity of the DCs and that this effect could be alleviated by the RNAi-mediated knockdown of TRPV2 but, importantly, not by the pharmacological (antagonists) or molecular (RNAi) suppression of TRPV1 and TRPV4 activities/levels. Likewise, the heat shock-induced robust membrane currents were selectively and markedly inhibited by TRPV2 “silencing” whereas modulation of TRPV1 and TRPV4 activities, again, had no effect. These intriguing data introduce TRPV2-coupled signaling as a key player in mediating the cellular actions of heat shock on DCs.
Journal: FEBS Letters - Volume 587, Issue 9, 2 May 2013, Pages 1440–1445