کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2048082 | 1074060 | 2013 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Sulfation of keratan sulfate proteoglycan reduces radiation-induced apoptosis in human Burkitt’s lymphoma cell lines Sulfation of keratan sulfate proteoglycan reduces radiation-induced apoptosis in human Burkitt’s lymphoma cell lines](/preview/png/2048082.png)
This study focuses on clarifying the contribution of sulfation to radiation-induced apoptosis in human Burkitt’s lymphoma cell lines, using 3′-phosphoadenosine 5′-phosphosulfate transporters (PAPSTs). Overexpression of PAPST1 or PAPST2 reduced radiation-induced apoptosis in Namalwa cells, whereas the repression of PAPST1 expression enhanced apoptosis. Inhibition of PAPST slightly decreased keratan sulfate (KS) expression, so that depletion of KS significantly increased radiation-induced apoptosis. In addition, the repression of all three N-acetylglucosamine-6-O-sulfotransferases (CHST2, CHST6, and CHST7) increased apoptosis. In contrast, PAPST1 expression promoted the phosphorylation of p38 MAPK and Akt in irradiated Namalwa cells. These findings suggest that 6-O-sulfation of GlcNAc residues in KS reduces radiation-induced apoptosis of human Burkitt’s lymphoma cells.
► Overexpression of PAPST reduced radiation-induced apoptosis in Namalwa cells.
► Inhibition of PAPST slightly decreased the expression of KS.
► Depletion of KS significantly increased radiation-induced apoptosis.
► The repression of N-acetylglucosamine-6-O-sulfotransferases increased apoptosis.
► PAPST1 expression promoted the phosphorylation of p38 MAPK and Akt in irradiated cells.
Journal: FEBS Letters - Volume 587, Issue 2, 16 January 2013, Pages 231–237