کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2048126 1074063 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biosynthesis of the pyoverdine siderophore of Pseudomonas aeruginosa involves precursors with a myristic or a myristoleic acid chain
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Biosynthesis of the pyoverdine siderophore of Pseudomonas aeruginosa involves precursors with a myristic or a myristoleic acid chain
چکیده انگلیسی

Pyoverdine I (PVDI) is the major siderophore produced by Pseudomonas aeruginosa to import iron. Biosynthesis of this chelator involves non-ribosomal peptide synthetases and other enzymes. PvdQ is a periplasmic enzyme from the NTN hydrolase family and is involved in the final steps of PVDI biosynthesis. A pvdQ mutant produces two non-fluorescent PVDI precursors with a higher molecular mass than PVDI. In the present study, we describe the use of mass spectrometry to determine the structure of these PVDI precursors and show that they both contain a unformed chromophore like ferribactin, and either a myristic or myristoleic chain that must be removed before PVDI is secreted into the extracellular medium.


► We solve by mass spectrometry the structure of two pyoverdine siderophore precursors.
► A PvdQ mutant produces these precursors.
► They both have a higher molecular mass as the pyoverdine.
► We show that both compounds contain an unformed chromophore and a myristic or a myristoleic acid chain.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 586, Issue 1, 2 January 2012, Pages 96–101
نویسندگان
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