Induction of epithelial-mesenchymal transition with O-glycosylated oncofetal fibronectin

Epithelial-mesenchymal transition (EMT) has been shown to play a key role in embryogenesis and cancer progression. We previously found that fibronectin (FN) carrying O-GalNAc at a specific site is selectively expressed in cancer and fetal cells/tissues, and termed oncofetal FN (onfFN). Here, we show that (i) a newly-established monoclonal antibody against FN lacking the O-GalNAc, termed normalFN (norFN), is useful for isolation of onfFN, (ii) onfFN, but not norFN, can induce EMT in human lung carcinoma cells, (iii) onfFN has a synergistic effect with transforming growth factor (TGF)β1 in EMT induction.

► A new hybridoma for an isoform of fibronectin (FN) was established.
► FN carrying O-GalNAc, expressed in fetal and cancer cells, was isolated.
► FN carrying O-GalNAc could induce epithelial-mesenchymal transition.