کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2048333 1074076 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cyclin-dependent kinase 4 is a novel target in micoRNA-195-mediated cell cycle arrest in bladder cancer cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Cyclin-dependent kinase 4 is a novel target in micoRNA-195-mediated cell cycle arrest in bladder cancer cells
چکیده انگلیسی

miRNAs are a class of small-noncoding RNAs capable of negatively regulating gene expression. Here, we found that miR-195 is down-regulated in human bladder cancer tissue versus normal adjacent tissue. To better characterize the role of miR-195 in bladder cancer, we conducted gain of function analysis by transfecting bladder cancer cell line T24 with chemically synthesized miR-195 mimic. We identified CDK4, an early G1 cell cycle regulator, as a novel target of miR-195. Selective over-expression of miR-195 could induce G1-phase arrest in T24 cells, and subsequently inhibit T24 cell growth. These findings indicate that miR-195 could be a potential tumor suppressor in bladder cancer.


► miR-195 is down-regulated in bladder cancer tissue samples.
► miR-195 inhibits bladder cancer cell T24 growth both in vitro and in vivo.
► miR-195 could induce T24 cell cycle arrest by suppressing CDK4.
► CDK4 is a direct target of miR-195.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 586, Issue 4, 17 February 2012, Pages 442–447
نویسندگان
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