کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2048412 1074079 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
“The preadipocyte factor” DLK1 marks adult mouse adipose tissue residing vascular cells that lack in vitro adipogenic differentiation potential
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
“The preadipocyte factor” DLK1 marks adult mouse adipose tissue residing vascular cells that lack in vitro adipogenic differentiation potential
چکیده انگلیسی

Delta-like 1 (Dlk1) is expressed in 3T3-L1 preadipocytes and has frequently been referred to as “the” preadipocyte marker, yet the phenotype of DLK1+ cells in adipose tissue remains undetermined. Herein, we demonstrate that DLK1+ cells encompass around 1–2% of the adult mouse adipose stromal vascular fraction (SVF). Unexpectedly, the DLK1+SVF population was enriched for cells expressing genes generally ascribed to the vascular lineage and did not possess any adipogenic differentiation potential in vitro. Instead, DLK1+ cells comprised an immediate ability for cobblestone formation, generation of tube-like structures on matrigel, and uptake of Acetylated Low Density-Lipoprotein, all characteristics of endothelial cells. We therefore suggest that DLK1+SVF cells are of a vascular origin and not them-selves committed preadipocytes as assumed hitherto.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 583, Issue 17, 3 September 2009, Pages 2947–2953
نویسندگان
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