کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2048497 | 1074082 | 2012 | 6 صفحه PDF | دانلود رایگان |
Rho-family small GTPases regulate epithelial cell sheet migration by organizing actin and myosin during wound healing. Here, we report that Pak3, but not Pak1, is a downstream target protein for Rac1 in wound closure of the Drosophila larval epidermis. Pak3-deficient larvae failed to close a wound hole and this defect was not rescued by Pak1 expression, indicating differential functions of the two proteins. Pak3 localized to the wound margin, which selectively required Rac1. Pak3-deficient larvae showed severe defects in actin-myosin organization at the wound margin and in submarginal cells, which was reminiscent of the phenotypes of Rac1-deficient larvae. These results suggest that Pak3 specifically mediates Rac1 signaling in organizing actin and myosin during Drosophila epidermal wound healing.
► Pak3 is required for Drosophila epidermal wound closure.
► Pak3 localizes to the wound margin and this process requires Rac1.
► Pak3-deficient larvae fail to properly organize actin and myosin at the wound margin.
Journal: FEBS Letters - Volume 586, Issue 6, 23 March 2012, Pages 772–777