کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2048569 1074084 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
SSO1450 – A CAS1 protein from Sulfolobus solfataricus P2 with high affinity for RNA and DNA
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
SSO1450 – A CAS1 protein from Sulfolobus solfataricus P2 with high affinity for RNA and DNA
چکیده انگلیسی

Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated protein genes (cas genes) are ubiquitous in archaea and eubacteria. It has been suggested that CRISPR and CAS proteins act as an immune system preventing the invasion of foreign genomic elements at the DNA level. The protein SSO1450 from Sulfolobus solfataricus (Sso) P2 belongs to the CAS1 cluster which is one of the core protein clusters most frequently associated with CRISPR sequences. In this study we show that SSO1450 is a high-affinity nucleic acid binding protein. It binds DNA, RNA and DNA–RNA hybrid apparently sequence non-specific in a multi-site binding mode. Furthermore, SSO1450 promotes the hybridization of complementary nucleic acid strands.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 583, Issue 12, 18 June 2009, Pages 1928–1932
نویسندگان
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