کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2048803 1074101 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A disulfide linkage in a CCCH zinc finger motif of an Arabidopsis CPSF30 ortholog
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
A disulfide linkage in a CCCH zinc finger motif of an Arabidopsis CPSF30 ortholog
چکیده انگلیسی

The Arabidopsis ortholog of the 30 kDa subunit of the cleavage and polyadenylation factor (AtCPSF30) is an RNA binding endonuclease, and the endonuclease activity is inhibited by reducing agents. Here, we report the presence of a disulfide linkage in the endonuclease motif based on comparative mass spectrometry (MS) analysis of reduced and non-reduced but carbamidomethylated protein. This analysis reveals that this disulfide bond involves a CCCH zinc finger motif, one that is associated with the endonuclease activity of AtCPSF30. This finding raises the possibility that redox regulation of AtCPSF30 may occur through oxidation and reduction of the disulfide linkage.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 584, Issue 21, 5 November 2010, Pages 4408–4412
نویسندگان
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