کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2048809 | 1074101 | 2010 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Studying base pair open–close kinetics of tRNALeu by TROSY-based proton exchange NMR spectroscopy
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The millisecond conformational flexibility is functionally important for nucleic acids and can be studied through probing the base pair open–close kinetics by proton exchange nuclear magnetic resonance (NMR) spectroscopy. Here, the traditional imino proton exchange NMR experiments were modified with transverse relaxation optimized spectroscopy and were applied to accurately measure imino proton exchange rates of all base pairs in Escherichia coli tRNALeu (CAG), and their dependence on magnesium ion concentration. Finally, we correlated millisecond conformational flexibility with aminoacylation of tRNALeu and proposed that the flexibility of the acceptor stem and the core region might contribute to aminoacylation of tRNALeu.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 584, Issue 21, 5 November 2010, Pages 4449–4452
Journal: FEBS Letters - Volume 584, Issue 21, 5 November 2010, Pages 4449–4452
نویسندگان
Zhan-Xi Hao, Min Tan, Chang-Dong Liu, Rui Feng, En-Duo Wang, Guang Zhu,