کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2048904 1074106 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A bacterial ortholog of class II lysyl-tRNA synthetase activates lysine
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
A bacterial ortholog of class II lysyl-tRNA synthetase activates lysine
چکیده انگلیسی

Aminoacyl-tRNA synthetases produce aminoacyl-tRNAs, essential substrates for accurate protein synthesis. Beyond their central role in translation some of these enzymes or their orthologs are recruited for alternative functions, not always related to their primary cellular role. We investigate here the enzymatic properties of GenX (also called PoxA and YjeA), an ortholog of bacterial class II lysyl-tRNA synthetase. GenX is present in most Gram-negative bacteria and is homologous to the catalytic core of lysyl-tRNA synthetase, but it lacks the amino terminal anticodon binding domain of the latter enzyme. We show that, in agreement with its well-conserved lysine binding site, GenX can activate in vitro l-lysine and lysine analogs, but does not acylate tRNALys or other cellular RNAs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 584, Issue 14, 16 July 2010, Pages 3055–3060
نویسندگان
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