کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2049113 | 1074117 | 2008 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To understand physiological roles of tissue mast cells, we established a culture system where bone marrow-derived immature mast cells differentiate into the connective tissue-type mast cell (CTMC)-like cells through modifying the previous co-culture system with Swiss 3T3 fibroblasts. Our system was found to reproducibly mimic the differentiation of CTMCs on the basis of several criteria, such as granule maturation and sensitivity to cationic secretagogues. The gene expression profile obtained by the microarray analyses was found to reflect many aspects of the differentiation. Our system is thus helpful to gain deeper insights into terminal differentiation of CTMCs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 582, Issue 10, 30 April 2008, Pages 1444–1450
Journal: FEBS Letters - Volume 582, Issue 10, 30 April 2008, Pages 1444–1450
نویسندگان
Hirotsugu Takano, Shunsuke Nakazawa, Yasushi Okuno, Naritoshi Shirata, Sohken Tsuchiya, Takayuki Kainoh, Seigoh Takamatsu, Kazuyuki Furuta, Yoshitaka Taketomi, Yuko Naito, Hiromu Takematsu, Yasunori Kozutsumi, Gozoh Tsujimoto, Makoto Murakami,