کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2049191 | 1074119 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular recognition of single-stranded RNA: Neomycin binding to poly(A)
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular recognition of single-stranded RNA: Neomycin binding to poly(A) Molecular recognition of single-stranded RNA: Neomycin binding to poly(A)](/preview/png/2049191.png)
چکیده انگلیسی
Poly(A) is a relevant sequence in cell biology due to its importance in mRNA stability and translation initiation. Neomycin is an aminoglycoside antibiotic that is well known for its ability to target various nucleic acid structures. Here it is reported that neomycin is capable of binding tightly to a single-stranded oligonucleotide (A30) with a Kd in the micromolar range. CD melting experiments support complex formation and indicate a melting temperature of 47 °C. The poly(A) duplex, which melts at 44 °C (pH 5.5), was observed to melt at 61 °C in the presence of neomycin, suggesting a strong stabilization of the duplex by the neomycin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 583, Issue 13, 7 July 2009, Pages 2269–2275
Journal: FEBS Letters - Volume 583, Issue 13, 7 July 2009, Pages 2269–2275
نویسندگان
Hongjuan Xi, David Gray, Sunil Kumar, Dev P. Arya,