A mammalian insulysin homolog is regulated by enzyme IIAGlc of the glucose transport system in Vibrio vulnificus

Vibrio vulnificus is an opportunistic human pathogen that causes severe infections in susceptible individuals. While the components of the Escherichia coli phosphoenolpyruvate: sugar phosphotransferase system (PTS) have been shown to regulate numerous targets, little such information is available for the V. vulnificus PTS. Here we show that enzyme IIAGlc of the PTS regulates the peptidase activity of a mammalian insulysin homolog in V. vulnificus. While interaction of IIAGlc with the insulysin homolog is independent of the phosphorylation state of IIAGlc, only unphosphorylated IIAGlc activates the insulysin homolog. Taken together, our results suggest that the V. vulnificus insulysin-IIAGlc complex plays a role in survival in the host by sensing glucose.Structured summaryMINT-8045996: IIA glu (uniprotkb:Q7MBY2) binds (MI:0407) to vIDE (uniprotkb:Q7MIS6) by pull down (MI:0096)MINT-8045817, MINT-8045967: IIA glu (uniprotkb:Q7MBY2) physically interacts (MI:0915) with vIDE (uniprotkb:Q7MIS6) by pull down (MI:0096)