Keywords: TFA، اسید trifluoroacetic; AN, acetonitrile; AVG, average; EDC, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide; DMAP, N,N-dimethyl-4-aminopyridine; TEA, triethylamine; TFA, trifluoroacetic acid; LH, light-harvesting; RC, reaction center; Chl, chlorophyll; BChl, bacteriochlorophyll;
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Keywords: TFA، اسید trifluoroacetic; PFBA, perfluorobenzoic acid; PFBSA, perfluorobenzenesulfonic acid; DMAc, N,N-dimethylacetamide; DMSO, dimethyl sulfoxide; TFA, trifluoroacetic acid; GC–MS, gas chromatography-mass spectrometry; TLC, thin layer chromatographyPerfluorobenzoic acid; Perfluor
Keywords: TFA، اسید trifluoroacetic; AcOH, acetic acid; ACN, acetonitrile; c-SCX, chiral strong cation exchanger; DEA, diethyl amine; FA, formic acid; MeOH, methanol; SA, selectand (analyte); SO, selector; TEA, triethyl amine; TFA, trifluoroacetic acid; ZWIX, zwitterion ion exchange-type sta
Keywords: TFA، اسید trifluoroacetic; AFM, atomic force microscopy; ATR, attenuated total reflectance; DSC, differential scanning calorimetry; FTIR, Fourier transform infrared; PVA, polyvinyl alcohol; RH, relative humidity; SEM, scanning electron microscopy; TFA, trifluoroacetic acid; Tg, gla
Keywords: TFA، اسید trifluoroacetic; DSC, differential scanning calorimetry; HPLC, high performance liquid chromatography; PBS, phosphate buffered saline; PVA, poly(vinyl alcohol); R.H., relative humidity; SEM, scanning electron microscopy; TFV, tenofovir; TFA, trifluoroacetic acid; XRD, X-r
Keywords: TFA، اسید trifluoroacetic; Tryptophan; Recombinant monoclonal antibody; Fluorescence detection; Mass spectrometryUV, ultraviolet; DTT, dithiothreitol; TFA, trifluoroacetic acid; HPLC, high-performance liquid chromatography; LC–MS, liquid chromatography–mass spectrometry; MS/MS, tan
Keywords: TFA، اسید trifluoroacetic; Ubiquitination; Gly-Gly enrichment; Enrichment efficiencyBoc-Gly-Gly-NHS, (tert-butoxycarbonyl) glycylglycine; Gly-Gly, di-glycine; T3P®, propylphosphonic anhydride solution; DMSO, dimethyl sulfoxide; TFA, trifluoroacetic acid; LC, liquid chromatography;
Keywords: TFA، اسید trifluoroacetic; Recombinant monoclonal antibodies; Methionine oxidation; Deglycosylation; Limited proteolytic digestion; Stable isotope; Mass spectrometryDSC, differential scanning calorimetry; LC–MS, liquid chromatography–mass spectrometry; TFA, trifluoroacetic acid; DT
Microwave-assisted extraction of cyclotides from Viola ignobilis
Keywords: TFA، اسید trifluoroacetic; Viola ignobilis; Violaceae; Microwave-assisted extraction; Vigno cyclotides; CyclopeptidesMAE, microwave-assisted extraction; SPE, solid-phase extraction; HPLC, high-performance liquid chromatography; TFA, trifluoroacetic acid; UV–VIS, ultraviolet–visible
Impact of cofactor-binding loop mutations on thermotolerance and activity of E. coli transketolase
Keywords: TFA، اسید trifluoroacetic; GA, glycolaldehyde; HPA, hydroxypyruvic acid; TFA, trifluoroacetic acid; TPP, thiamine diphosphate; TK, transketolaseBiocatalysis; Thermostability; Thermo-inactivation; Protein engineering; Cofactor-binding loops; Transketolase
In vitro angiotensin I converting enzyme inhibition by a peptide isolated from Chiropsalmus quadrigatus Haeckel (box jellyfish) venom hydrolysate
Keywords: TFA، اسید trifluoroacetic; Chiropsalmus quadrigatus; Box jellyfish; ACE; Hypertension; VenomJVE, jellyfish venom extract; EH, enzymatic hydrolysate; TFA, trifluoroacetic acid; RP-HPLC, reverse phase-high performance liquid chromatography; MALDI-TOF/MS, Matrix-assisted laser desorpt
Synthetic peptides for the immunodiagnosis of hepatitis A virus infection
Keywords: TFA، اسید trifluoroacetic; NMP, N-Methyl-2-pyrrolidone; HOBt, hydroxybenzotriazole; DIC, N,N′-Diisopropylcarbodiimide; TFA, trifluoroacetic acid; DCM, dichloromethane; TIPS, triisopropylsilane; TBTU, 2-(1H-Benzotriazol-1-yl)-1,1,3,3-tetramethyluroniumtetrafluoroborate; DMSO, dimeth
Shell proteome of rhynchonelliform brachiopods
Keywords: TFA، اسید trifluoroacetic; ACN, acetonitrile; DC-LMR®, rapid DeCalcifying solution; DTT, DL-dithiothreitol; f, fiber; GO, gene ontology; gr, granule; i.m., insoluble matrix; p.l., primary layer; s.l., secondary layer; TFA, trifluoroacetic acid; t.l., tertiary layerBrachiopods; Shel
Synthesis and pharmacological evaluation of benzannulated derivatives as potent and selective sigma-1 protein ligands
Keywords: TFA، اسید trifluoroacetic; Sigma 1; Alzheimer; Benzazolinone; Benzimidazole; BenzimidazoloneDCM, dichloromethane; DMEM, Dulbecco's Modified Eagle Medium; DMSO, dimethylsulphoxide; PHPLC, purity determined by HPLC; TLC, thin layer chromatography; TFA, trifluoroacetic acid; tR, HPLC
Bio-analytical method based on MALDI-MS analysis for the quantification of CIGB-300 anti-tumor peptide in human plasma
Keywords: TFA، اسید trifluoroacetic; MALDI-TOF MS, Matrix Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry; QCs, quality control samples; CSs, calibration standard samples; TFA, trifluoroacetic acid; IS, internal standard; LLOQ, lower limit of quantification; AUC, area u
Low temperature and water dehydration increase the levels of asiaticoside and madecassoside in Centella asiatica (L.) Urban
Keywords: TFA، اسید trifluoroacetic; mg.g− 1 fw, milligram per gram fresh weight; As, asiaticoside; Ms, madecassoside; Aa, asiatic acid; Ms + B, madecassoside and its isomer, asiaticoside B; TFA, trifluoroacetic acid; P.A.R., photosynthetically active radiationCentella asiatica (L.) Urban; A
Discovery and structure–activity relationships of pyrazolodiazepine derivatives as the first small molecule agonists of the Drosophila sex peptide receptor
Keywords: TFA، اسید trifluoroacetic; SPR, sex peptide receptor; SP, sex peptide; MIPs, myoinhibitory-peptides; Drm, Drosophila melanogaster; TEA, triethyl amine; TFA, trifluoroacetic acid; EDC, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide; DMF, dimethylformamide; DCM, dichloromethane; HOBt,
Impact of in vitro simulated digestion on the potential health benefits of proanthocyanidins from Choerospondias axillaris peels
Keywords: TFA، اسید trifluoroacetic; PAs, Proanthocyanidins; C. axillaris, Choerospondias axillaris; TPC, Total polyphenol content; mDP, Mean degree of polymerization; LC–MSn, Liquid chromatography–mass-spectrometry; TFA, Trifluoroacetic acid; EC, Epicatechin; ABTS, 2, 2′-azino-bis (3-ethylb
Structure of Hepatitis C Virus Envelope Glycoprotein E1 Antigenic Site 314–324 in Complex with Antibody IGH526
Keywords: TFA، اسید trifluoroacetic; HCV, hepatitis C virus; mAb, monoclonal antibody; MD, molecular dynamics; DMF, N,N-dimethylformamide; TFA, trifluoroacetic acid; APS, Advanced Photon Source; vdW, van der WaalsHCV; E1 envelope glycoprotein; IGH526; vaccine design
Signal loss due to oligomerization in ELISA analysis of amyloid-beta can be recovered by a novel sample pre-treatment method
Keywords: TFA، اسید trifluoroacetic; Sample pre-treatment for amyloid-beta ELISA analysisAlzheimer’s disease; Amyloid-beta; ELISA; Sample pre-treatment; Oligomers; Steric hindranceAD, Alzheimers disease; Aβ, amyloid-beta; TFA, trifluoroacetic acid; HFIP, hexafluoroisopropanol; FA, formic aci
Trypanosoma cruzi chemical proteomics using immobilized benznidazole
Keywords: TFA، اسید trifluoroacetic; ROS, reactive oxygen species; MALDI-TOF/TOF MS, matrix assisted laser desorption ionization-time of flight–time of flight-mass spectrometry; NMR, nuclear magnetic resonance; TLC, thin layer chromatography; TFA, trifluoroacetic acid; MS, mass spectrometry;
Reversible protection of Cys-93(β) by PEG alters the structural and functional properties of the PEGylated hemoglobin
Keywords: TFA، اسید trifluoroacetic; Hb, hemoglobin; PEG, polyethylene glycol; PEGylation, conjugation with polyethylene glycol; PDPH, 3-(2-pyridyldithio)propionyl hydrazide; PBS, phosphate buffered saline; TFA, trifluoroacetic acid; HBOC, hemoglobin based oxygen carrier; P50, partial oxygen
HPLC method development, validation and impurity characterization for an antitumor Hsp90 inhibitor—PU-H71 (NSC 750424)
Keywords: TFA، اسید trifluoroacetic; PU-H71; NSC 750424; Forced degradation; HPLC validation; Stability study; Impurity characterizationHsp, heat shock protein; ATP, adenosine triphosphate; ADP, adenosine diphosphate; TFA, trifluoroacetic acid; ACN, acetonitrile; PDA, photodiode array; RRT,
Extracellular peptidases of imaginal discs of Drosophila melanogaster
Keywords: TFA، اسید trifluoroacetic; TFA, trifluoroacetic acid; RP-HPLC, reversed phase-high performance liquid chromatography; AMC, 7-amino-4-methylcoumarin; DPP IV, dipeptidyl peptidase IV; ACE, angiotensin-converting enzyme; Ance, Drosophila melanogaster angiotensin-converting enzyme; Ace
Tailored protein release from biodegradable poly(ε-caprolactone-PEG)-b-poly(ε-caprolactone) multiblock-copolymer implants
Keywords: TFA، اسید trifluoroacetic; PEG, poly (ethylene glycol); PCL, poly (ε-caprolactone); PDLA, poly(dl-lactide); PLGA, poly(dl-lactide-co-glycolide); BSA, bovine serum albumin; Lys, lysozyme; Gos, goserelin; Ins, insulin; CA, carbonic anhydrase; TFA, trifluoroacetic acid; CDCL3, deutera
Assessment of pre-analytical and analytical variables affecting peptidome profiling of gingival crevicular fluid by MALDI-TOF mass spectrometry
Keywords: TFA، اسید trifluoroacetic; MALDI-TOF, matrix-assisted laser desorption ionization-time-of-flight; MS, mass spectrometry; GCF, gingival crevicular fluid; PPs, paper points; PSs, paper strips; PIC, protease inhibitor cocktail; TFA, trifluoroacetic acid; ACN, acetonitrile; AcAc, aceti
Poly(γ-benzyl-l-glutamate)-PEG-alendronate multivalent nanoparticles for bone targeting
Keywords: TFA، اسید trifluoroacetic; HAP, hydroxyapatite; PBLG, poly(γ-benzyl-l-glutamate); PEG, polyethylene glycol; DMF, dimethylformamide; BLG-NCA, γ-benzyl-l-glutamate-N-carboxylic anhydride; TFA, trifluoroacetic acid; MeO-PEG5k-NH2, α-methoxy-ω-amino poly(ethylene glycol)5000, NHSPEG6k-
CYP1A1 and CYP1B1-Mediated Biotransformation of the Antitrypanosomal Methamidoxime Prodrug DB844 Forms Novel Metabolites Through Intramolecular Rearrangement
Keywords: TFA، اسید trifluoroacetic; CYP1A1; CYP1B1; cytochrome P450; intramolecular rearrangement; drug metabolism; prodrugs; human African trypanosomiasis; nitric oxide; CID, collision-induced dissociation; CNS, central nervous system; CYP, cytochrome P450; HAT, human African trypanosomias
Fluorogenic sialic acid glycosides for quantification of sialidase activity upon unnatural substrates
Keywords: TFA، اسید trifluoroacetic; 4MU, 4-methylumbelliferyl; TFA, trifluoroacetic acid; t-Boc, tert-butoxycarbonyl; HBTU, O-(benzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate; DANA, 2-dehydroxy-2-deoxyneuraminic acidSialidases; Neuraminidases; 4-MU; Sialic acid derivativ
Antimicrobial and phytochemical properties of stem bark extracts from Piptadeniastrum africanum (Hook f.) Brenan
Keywords: TFA، اسید trifluoroacetic; WE, water extract; ME, methanolic extract; EAE, ethyl acetate extract; TFA, trifluoroacetic acid; TSB, Tryptone Soya Broth; ISB, Iso Sensitest Broth; SAB, Sabouraud dextrose agar; PDA, Potato Dextrose Agar; MIC, minimum inhibitory concentration; MBC, mini
The structure and immunoreactivity of exopolysaccharide isolated from Lactobacillus johnsonii strain 151
Keywords: TFA، اسید trifluoroacetic; DEAE, diethylaminoethyl; EPS, exopolysaccharide; LAB, lactic acid bacteria; TCA, trichloroacetic acid; TFA, trifluoroacetic acid; TMB, 3,3′,5,5′-tetramethylbenzidineLactobacillus; Exopolysaccharide structure; Immunoreactivity; NMR
LC/MS analysis of proteolytic peptides in wheat extracts for determining the content of the allergen amylase/trypsin inhibitor CM3: Influence of growing area and variety
Keywords: TFA، اسید trifluoroacetic; CM, chloroform/methanol soluble; LC, liquid chromatography; LTP, lipid transfer protein; MS, mass spectrometry; RP, reverse phase; SDS–PAGE, sodium dodecyl sulphate–polyacrylamide gel electrophoresis; TFA, trifluoroacetic acid; TIS, triisopropylsilaneBake
Vibrational circular dichroism study of solvent- and temperature-induced conformational changes in poly-γ-benzyl-l-glutamate and poly-β-benzyl-l-aspartate
Keywords: TFA، اسید trifluoroacetic; PBLG, poly-γ-benzyl-l-glutamate; PBLA, poly-β-benzyl-l-aspartate; TFA, trifluoroacetic acid; IR, infrared; VCD, vibrational circular dichroism; ECD, electronic circular dichroism; CHCl3, trichloromethane; PPII, polyproline IIPolypeptide; Poly-γ-benzyl-l-g
Inhibition of protein glycation by urea and thiourea derivatives of glycine/proline conjugated benzisoxazole analogue – Synthesis and structure–activity studies
Keywords: TFA، اسید trifluoroacetic; Amino acids; Conjugation; Urea/thiourea; AntiglycationBoc, t-butoxycarbonyl; EDCI, 1-(3-dimethylaminopropyl)-3-ethyl-carbodiimide; HOBt, 1-hydroxybenzotriazole; NMM, N-methyl morpholine; TFA, trifluoroacetic acid
Dephosphorylation of intact glycoprotein to greatly improve digestion efficiency coupled with matrix-assisted laser desorption/ionization–Fourier transform ion cyclotron resonance mass spectrometric analysis
Keywords: TFA، اسید trifluoroacetic; PTM(s), post translational modification(s); DHB, 2,5-dihydroxylbenzoic acid; ACN, acetonitrile; TFA, trifluoroacetic acid; MALDI-FTICR MS, matrix-assisted laser desorption/ionization–Fourier transform ion cyclotron resonance mass spectrometry; DTT, dithio
A novel Aurora-A-mediated phosphorylation of p53 inhibits its interaction with MDM2
Keywords: TFA، اسید trifluoroacetic; IMAC, immobilized metal affinity chromatography; GST, glutathione S-transferase; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; RIPA buffer, radioimmune precipitation assay buffer; DTT, 1,4-dithiothreitol; TFA, trifluoroacetic acid;
Preservation of saffron floral bio-residues by hot air convection
Keywords: TFA، اسید trifluoroacetic; TFA, trifluoroacetic acid; HCl, hydrochloridric acid; Cab∗, chroma; hab, hue; ΔE∗, total color differences; TPI, Total Phenolic Index; D3*5-diG, delphinidin 3,5-di-O-glucoside; P3*5-diG, petunidin 3*5-di-O-glucoside; D3-G, delphinidin 3-O-glucoside; M3*5-
IgG2 disulfide isoform conversion kinetics
Keywords: TFA، اسید trifluoroacetic; HPLC, high performance liquid chromatography; TFA, trifluoroacetic acid; PBS, phosphate buffered saline; NEM, N-ethyl maleimide; UV, ultraviolet; Redox, oxidation–reductionDisulfide shuffling; Antibodies; Physiological models; Redox reactions; Blood chemi
Synthesis and cellular studies of polyamine conjugates of a mercaptomethyl–carboranylporphyrin
Keywords: TFA، اسید trifluoroacetic; PDT, photodynamic therapy; BNCT, boron neutron capture therapy; TPPF, 5,10,15,20-tetrakis(2,3,4,5,6-pentafluorophenyl)porphyrin; DMSO, dimethylsulfoxide; THF, tetrahydrofuran; TFA, trifluoroacetic acid; LET, linear energy transfer; PBS, phosphate buffered
Inflammatory, immune and lipid transportation proteins are differentially expressed in spontaneous and proximal deep vein thrombosis patients
Keywords: TFA، اسید trifluoroacetic; VTE, Venous Thromboembolism; PE, Pulmonary Embolism; DVT, Deep Vein Thrombosis; MPS, Microparticles; PPP, Platelet Poor Plasma; DTT, Dithiothreitol; ACN, Acetonitrile; TFA, Trifluoroacetic Acid; SCX, Cation Exchange; UPLC, Ultra Performance Liquid Chromat
Solving the ‘Brown snake paradox’: In vitro characterisation of Australasian snake presynaptic neurotoxin activity
Keywords: TFA، اسید trifluoroacetic; CBCNM, chick biventer cervicis nerve-muscle; CCh, carbachol; HPLC, high performance liquid chromatography; TFA, trifluoroacetic acid; ACN, acetonitrile‘Brown snake paradox’; Textilotoxin; Taipoxin; Pseudonaja textilis; Oxyuranus scutellatus scutellatus; P
Partial characterization and immunomodulatory activity of polysaccharides from the stem of Dendrobium officinale (Tiepishihu) in vitro
Keywords: TFA، اسید trifluoroacetic; DOP, crude Dendrobium officinale polysaccharide; DOP-1, the first fraction of DOP; DOP-2, the second fraction of DOP; NK, natural killer; NO, nitric oxide; TFA, trifluoroacetic acid; HPLC, high performance liquid chromatography; PBS, phosphate buffered sa
Development and application of high-performance liquid chromatography for the study of two new oxyprenylated anthraquinones produced by Rhamnus species
Keywords: TFA، اسید trifluoroacetic; CV, coefficient of variation; LOD, limit of detection; LOQ, limit of quantification; ODS, octadecylsilane; QC, quality control; TFA, trifluoroacetic acid; ULOQ, upper limit of quantificationExtraction procedure; HPLC–UV/vis; Method development; Oxyprenyla
A novel in-situ enhanced blasting extrusion technique — Extrudate analysis and optimization of processing conditions with okara
Keywords: TFA، اسید trifluoroacetic; OKP, okara powder; DF, dietary fiber; SDF, soluble dietary fiber; IDF, insoluble dietary fiber; TFA, trifluoroacetic acid; NS, neutral sugar; UA, uronic acid; RSM, response surface methodology; BBD, Box–Behnken design; SEM, scanning electron micrographs;
Characterization of transferrin glycopeptide structures in human cerebrospinal fluid
Keywords: TFA، اسید trifluoroacetic; CSF, cerebrospinal fluid; Tf, transferrin; TFA, trifluoroacetic acid; ppm, parts per million; MS, mass spectrometry; MS/MS, MSn tandem mass spectrometry; VWM, vanishing white matter; 2-DE, two-dimensional gel electrophoresis; min, minute(s); Da, Dalton; m
Synthesis and characterization of novel square planar copper(II)–dipeptide–1,10-phenanthroline complexes: Investigation of their DNA binding and cleavage properties
Keywords: TFA، اسید trifluoroacetic; EDCI, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide); HOBt, hydroxybenzotriazole; TFA, trifluoroacetic acid; His-trp, histidyl-tryptophan; His-tyr, histidyl-tyrosine; phen, 1,10-phenathroline; CT-DNA, calf-thymus DNA; Tris, Tris(hydroxymethyl)aminomethane
Protein quantitation using various modes of high performance liquid chromatography
Keywords: TFA، اسید trifluoroacetic; API, active pharmaceutical ingredient; CME, cellulose mixed ester; mAb, monoclonal antibody; nSEC, Non Ideal size exclusion chromatography; pI, isoelectric point; SAX, strong anion exchange; TFA, trifluoroacetic acid; tR, retention time; WCX, weak cation
Synthesis of uriedo and thiouriedo derivatives of peptide conjugated heterocycles – A new class of promising antimicrobials
Keywords: TFA، اسید trifluoroacetic; Synthesis; Derivatives of conjugates; Uriedo and thiouriedo; Potent antimicrobialsBoc, t-Butoxycarbonyl; NMM, N-Methyl morpholine; TFA, Trifluoroacetic acid
Optimization of mass spectral features in MALDI-TOF MS profiling of Acinetobacter species
Keywords: TFA، اسید trifluoroacetic; MALDI-TOF MS, Matrix-Assisted Laser Desorption Ionization-Time-Of-Flight Mass Spectrometry; CHCA, alpha-cyano-4-hydroxycinnamic acid; DHB, 2,5-dihydroxybenzoic acid; SA, sinapinic acid; FerA, ferulic acid; TFA, trifluoroacetic acid; a.u., arbitrary unitsA
Determination of time-dependent accumulation of d-lactate in the streptozotocin-induced diabetic rat kidney by column-switching HPLC with fluorescence detection
Keywords: TFA، اسید trifluoroacetic; DM, diabetes mellitus; TFA, trifluoroacetic acid; NBD-PZ, 4-nitro-7-piperazino-2,1,3-benzoxadiazole; I.S., internal standard; o-PD, o-phenylenediamine; 2-MQ, 2-methylquinoxaline; 5-MQ, 5-methylquinoxaline; AGEs, advanced glycation end productsd-Lactate; l