کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1220542 | 967788 | 2012 | 8 صفحه PDF | دانلود رایگان |
The crude polysaccharide from the stem of Dendrobium officinale (DOP) was obtained by water extraction and ethanol precipitation. Two fractions of DOP, DOP-1 and DOP-2, were fractioned by DEAE cellulose and Sephacryl S-400 gel filtration column chromatography. Their molecular weights and monosaccharide compositions were studied. The immunomodulatory activity of DOP, DOP-1 and DOP-2 was evaluated with in vitro cell models. The results showed that the 533.7 kDa DOP-1 and 159.5 kDa DOP-2 were mainly composed of mannose and glucose. Both DOP and its purified fractions significantly (P < 0.05) promoted splenocyte proliferation, enhanced natural killer cell-mediated cytotoxicity, and increased the phagocytosis and nitric oxide production of macrophages. Moreover, these two fractions caused a significant (P < 0.05) stimulation of cytokine secretion of both splenocytes and macrophages. These findings suggest that Dendrobium officinale polysaccharides should be explored as novel potential immunomodulators.
► The crude polysaccharide and its two fractions were isolated from the stem of D. officinale.
► Their molecular weight and monosaccharide compositions were studied.
► The immunomodulatory activity of three polysaccharides was evaluated with in vitro cell models.
► One fraction DOP-1 exhibited greater effects on lymphocyte activation.
► The other fraction DOP-2 was more effective on macrophage activation.
Journal: Journal of Functional Foods - Volume 4, Issue 1, January 2012, Pages 294–301