کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2049354 | 1074125 | 2010 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: FoxO1 represses LXRα-mediated transcriptional activity of SREBP-1c promoter in HepG2 cells FoxO1 represses LXRα-mediated transcriptional activity of SREBP-1c promoter in HepG2 cells](/preview/png/2049354.png)
Recent studies have demonstrated that FoxO1 modulates the expression of SREBP-1c, but the exact mechanism remains unknown. Our results demonstrate that FoxO1 suppresses the SREBP-1c promoter transcriptional activity in HepG2 cells. This repression was independent of FoxO1 binding to the SREBP-1c promoter, but LXR responsive elements (LXREs) were crucial to this phenomenon. Moreover, FoxO1 also strongly inhibited the LXRα-mediated elevated transcription by SREBP-1c promoter. Electrophoretic mobility shift assay and chromatin immuno-precipitation further suggested the ability of FoxO1 to inhibit LXRα binding with the LXRE in the SREBP-c promoter. FoxO1-mediated suppression of SREBP-1c promoter activity could be partially alleviated by insulin.
Journal: FEBS Letters - Volume 584, Issue 20, 22 October 2010, Pages 4330–4334