کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2049356 | 1074125 | 2010 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Ammonia permeability of the soybean nodulin 26 channel Ammonia permeability of the soybean nodulin 26 channel](/preview/png/2049356.png)
Soybean nodulin 26 (nod26), a member of the aquaporin superfamily, is the major protein component of the symbiosome membrane that encloses nitrogen-fixing bacteroids in root nodules. Previous work has demonstrated that nod26 facilitates the transport of water and glycerol, although a potential additional role as a channel for fixed ammonia efflux has been hypothesized. In the present study it is shown that recombinant nod26 reconstituted into proteoliposomes facilitates NH3 transport in an Hg2+-sensitive manner with a reduced activation energy, hallmarks of protein-facilitated transport characteristic of aquaporins. Comparison of the predicted single-channel transport rates of nod26 suggests a 4.9-fold preference for ammonia compared to water.
Journal: FEBS Letters - Volume 584, Issue 20, 22 October 2010, Pages 4339–4343