کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2049720 | 1074139 | 2008 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A reducing environment stabilizes HIF-2α in SH-SY5Y cells under hypoxic conditions A reducing environment stabilizes HIF-2α in SH-SY5Y cells under hypoxic conditions](/preview/png/2049720.png)
Accumulating evidence suggests that hypoxia-inducible factor-2 (HIF-2) is important for the cellular response to hypoxia. However, it is not clear how HIF-2 is regulated under hypoxic conditions. We investigated kinetic changes in redox status and HIF-2α accumulation in hypoxic SH-SY5Y cells. Our results demonstrated that hypoxia caused a reducing environment and increased HIF-2α protein levels. Experiments with redox modulations (N-acetylcysteine and l-buthionine sulfoximine) confirmed that a reducing environment induced HIF-2α accumulation while an oxidizing environment decreased it. In addition, experiments with SOD mimic, catalase, and exogenous H2O2 provided evidence that the presence of H2O2 down-regulated the amount of HIF-2α protein. This study offers novel evidence supporting redox status regulation of HIF-2α accumulation under hypoxic conditions.
Journal: FEBS Letters - Volume 582, Issue 28, 26 November 2008, Pages 3899–3902