کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2049902 1074146 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A single amino acid residue is responsible for species-specific incompatibility between CCT and α-actin
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
A single amino acid residue is responsible for species-specific incompatibility between CCT and α-actin
چکیده انگلیسی

Actin is dependent on the type-II chaperonin CCT (chaperonin containing TCP-1) to reach its native state. In vitro, yeast CCT folds yeast and also mammalian cytoplasmic (β/γ) actins but is now found to be incapable of folding mammalian skeletal muscle α-actin. Arrest of α-actin on yeast CCT at a folding cycle intermediate has been observed by electron microscopy. This discovery explains previous observations in vivo that yeast mutants expressing only the muscle actin gene are non-viable. Mutational analysis identified a single specific α-actin residue, Asn-297, that confers this species/isoform folding specificity. The implications of this incompatibility for chaperonin mechanism and actin–CCT co-evolution are discussed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 583, Issue 4, 18 February 2009, Pages 782–786
نویسندگان
, , , , , ,