کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2050002 | 1074150 | 2008 | 5 صفحه PDF | دانلود رایگان |
Methanothermobacter thermautotrophicus contains a multi-aminoacyl-tRNA synthetase complex (MSC) of LysRS, LeuRS and ProRS. Elongation factor (EF) 1A also associates to the MSC, with LeuRS possibly acting as a core protein. Analysis of the MSC revealed that LysRS and ProRS specifically interact with the idiosyncratic N- and C- termini of LeuRS, respectively. EF-1A instead interacts with the inserted CP1 proofreading domain, consistent with models for post-transfer editing by class I synthetases such as LeuRS. Together with previous genetic data, these findings show that LeuRS plays a central role in mediating interactions within the archaeal MSC by acting as a core scaffolding protein.Structured summaryMINT-6551032:EF1A (uniprotkb:O27132) physically interacts (MI:0218) with LeuRS (uniprotkb:O27552) by surface plasmon resonance (MI:0107)
Journal: FEBS Letters - Volume 582, Issue 15, 25 June 2008, Pages 2178–2182