کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2050145 | 1074156 | 2007 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Design and switch of catalytic activity with the DNAzyme–RNAzyme combination Design and switch of catalytic activity with the DNAzyme–RNAzyme combination](/preview/png/2050145.png)
Design and switch of catalytic activity in enzymology remains a subject of intense investigation. Here, we employ a DNAzyme–RNAzyme combination strategy for construction of a 10–23 deoxyribozyme-hammerhead ribozyme combination that targets different sites of the β-lactamase mRNA. The 10–23 deoxyribozyme-hammerhead ribozyme combination gene was cloned into phagemid vector pBlue-scriptIIKS (+). In vitro the single-strand recombinant phagemid vector containing the combination sequence exhibited 10–23 deoxyribozyme activity, and the linear transcript displayed hammerhead ribozyme activity. In bacteria, the 10–23 deoxyribozyme-hammerhead ribozyme combination inhibited the β-lactamase expression and repressed the growth of drug-resistant bacteria. Thus, we created a DNAzyme–RNAzyme combination strategy that provides a useful approach to design and switch of catalytic activities for nucleic acid enzymes.
Journal: FEBS Letters - Volume 581, Issue 9, 1 May 2007, Pages 1763–1768