کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2050439 | 1074170 | 2008 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Glutamate-induced glioma cell proliferation is prevented by functional expression of the glutamate transporter GLT-1 Glutamate-induced glioma cell proliferation is prevented by functional expression of the glutamate transporter GLT-1](/preview/png/2050439.png)
A tetracycline-dependent inducible system was used to achieve controlled expression of the glutamate transporter 1 (GLT-1) in C6 glioma cells. Non-induced cells show modest glutamate uptake and, in the presence of l-cystine, these cells tend to release substantial amounts of glutamate. Overnight exposure to doxycycline increased d-[3H]-aspartate uptake, reaching similar capacity as observed in cultured astrocytes. Efficient clearance of exogenously applied glutamate was evidenced in these cells, even in the presence of l-cystine. The addition of glutamate (100 μM) to the medium of non-induced cells significantly increased their proliferation rate, an effect that was blocked when the expression of GLT-1 was induced. This suggests that impaired glutamate uptake capacity in glioma cells indirectly contributes to their proliferation.
Journal: FEBS Letters - Volume 582, Issue 13, 11 June 2008, Pages 1847–1852