کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2050749 | 1074180 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evidence for targeting common siRNA hotspots and GC preference by plant Dicer-like proteins
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Evidence for targeting common siRNA hotspots and GC preference by plant Dicer-like proteins Evidence for targeting common siRNA hotspots and GC preference by plant Dicer-like proteins](/preview/png/2050749.png)
چکیده انگلیسی
Small interfering (si)RNAs isolated from Brassica juncea leaves infected by Turnip mosaic virus (TuMV) were characterized by cloning and sequencing. The TuMV siRNA population was dominated by 21 and 22-nt long species originated mainly from the same siRNA hotspots, indicating operational similarity between the plant Dicer-like (DCL) enzymes. Robust GC bias was observed for TuMV siRNAs versus the virus genome, indicating that DCL was more likely to target GC-rich regions. Furthermore, dicot micro-(mi)RNAs displayed higher GC% than their DCL1 substrate RNAs, implicating that the GC bias may be ancient, therefore may be important for the RNAi technology.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 581, Issue 17, 10 July 2007, Pages 3267–3272
Journal: FEBS Letters - Volume 581, Issue 17, 10 July 2007, Pages 3267–3272
نویسندگان
Thien Ho, Hui Wang, Denise Pallett, Tamas Dalmay,