کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2050783 | 1074181 | 2007 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of proliferating cell nuclear antigen (PCNA) isoforms in normal and cancer cells: There is no cancer-associated form of PCNA
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کلمات کلیدی
ABC, ammonium bicarbonate2D-PAGE, two-dimensional polyacrylamide gel electrophoresis - 2D-PAGE، الکتروفورز ژل پلی آکریل آمید دو بعدیCHAPS, 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate - CHAPS، 3 - [(3-Cholamidopropyl) dimetilammonio] -1-پروپان سولفوناتIEF, isoelectrofocusing - IEF، ایزو الکترو فوکوسIPG, immobilized pH gradient - IPG، gradient of immobilized pHACN, acetonitrile - استونیتریل
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Characterization of proliferating cell nuclear antigen (PCNA) isoforms in normal and cancer cells: There is no cancer-associated form of PCNA Characterization of proliferating cell nuclear antigen (PCNA) isoforms in normal and cancer cells: There is no cancer-associated form of PCNA](/preview/png/2050783.png)
چکیده انگلیسی
In order to clarify the status of PCNA in normal and transformed cells, we performed analysis of this protein by 2D-PAGE, Western blot and mass spectrometry. All the cell lines examined contained the major PCNA form (pI 4.57/30 kDa), that is not post-translationally modified. In addition to the major form, two minor isoforms (pI 4.52/30 kDa and pI 4.62/30 kDa) were also detected in all the cell lines examined. However, the level of PCNA in cancer cells is 5–6 folds higher than those in primary and most of the immortalized cells. Taken together, the significant difference in PCNA status between cancer and normal cells is not at the post-translational modifications but in the overall levels of PCNA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 581, Issue 25, 16 October 2007, Pages 4917–4920
Journal: FEBS Letters - Volume 581, Issue 25, 16 October 2007, Pages 4917–4920
نویسندگان
Stanislav N. Naryzhny, Hoyun Lee,