Keywords: IPG، gradient of immobilized pH; AD, atopic dermatitis; ADe, extrinsic type of AD; 2D-PAGE, two-dimensional polyacrylamide gel electrophoresis; MALDI-TOF, matrix-assisted laser desorption/ionization-time of flight; IPG, immobilized pH gradient; MARC, multiple affinity removal column; IEF
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Serum proteomic analyses for probing C3 fragment protein
Keywords: IPG، gradient of immobilized pH; AD, atopic dermatitis; ADe, extrinsic type of AD; ADi, intrinsic type of AD; 2D-PAGE, two-dimensional polyacrylamide gel electrophoresis; MALDI-TOF, matrix-assisted laser desorption/ionization-time of flight; IPG, immobilized pH gradient; MARC, multiple a
Barley germination: Spatio-temporal considerations for designing and interpreting ‘omics’ experiments
Keywords: IPG، gradient of immobilized pH; Barley; Germination; Malting; ProteomicsABA, abscisic acid; ABPP, activity-based protein profiling; BAC, bacterial artificial chromosome; ESI, electrospray ionization; EST, expressed sequence tag; GA, gibberellic acid; IPG, immobilized pH gradient; LC, li
2D DIGE proteomic analysis highlights delayed postnatal repression of α-fetoprotein expression in homocystinuria model mice
Keywords: IPG، gradient of immobilized pH; AFP, α-fetoprotein; CBS, cystathionine β-synthase; CTH, cystathionine γ-lyase; DTT, dithiothreitol; HCC, hepatocellular carcinoma; IEF, isoelectric focusing; IPG, immobilized pH gradient; MALDI-TOF/MS, matrix-assisted laser desorption/ionization-time of f
First proteome study of sporadic flowering in bamboo species (Bambusa vulgaris and Dendrocalamus manipureanus) reveal the boom is associated with stress and mobile genetic elements
Keywords: IPG، gradient of immobilized pH; DTT, dithiothreitol; CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate; BSA, bovine serum albumin; 2-DE, two dimensional gel electrophoresis; IPG, immobilized pH gradient; SDS-PAGE, sodium dodecyl sulphate polyacrylamide gel electrophoresis
Lobaplatin arrests cell cycle progression, induces apoptosis and alters the proteome in human cervical cancer cell Line CaSki
Keywords: IPG، gradient of immobilized pH; Lobaplatin; Cervical cancer; Proteome; Cell cycle; ApoptosisMTT, Methylthiazolyldiphenyl-tetrazolium bromide; TEMED, Tetramethyl ethylene diamine; SDS, Sodium dodecyl sulfate; Tris, Tris(hydroxymethyl)aminomethane; IPG, Immobilized PH gradient; PI, Propid
Technical variability of 2D gel electrophoresis – Application to soybean allergens
Keywords: IPG، gradient of immobilized pH; 2-DE, two-dimensional gel electrophoresis; CV, coefficient of variation expressed as percentage; GM, genetically modified; IEF, isoelectric focusing; IPG, immobilized pH gradient; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresisFalse po
Proteomics of old world camelid (Camelus dromedarius): Better understanding the interplay between homeostasis and desert environment
Keywords: IPG، gradient of immobilized pH; 2D, two-dimensional; MS, mass spectrometry; CHAPS, 3-(3-cholamidopropyl)-dimethylammoniopropane sulfonate; pI, isoelectric point; IPG, immobilized pH gradient; DTT, dithiothreitol; SDS, sodium dodecylsulfate; PAGE, polyacrylamide gel electrophoresis; TFA,
Proteomic analysis of “Moncada” mandarin leaves with contrasting fruit load
Keywords: IPG، gradient of immobilized pH; Alternate bearing; Citrus; Leaves; Primary metabolism; Proteomic; Redox stateCHAPS, 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate; 2D DIGE, two-dimensional difference gel electrophoresis; DTT, dithiothreitol; IPG, immobilized pH gradient; LC-
Isoelectric point optimization using peptide descriptors and support vector machines
Keywords: IPG، gradient of immobilized pH; FDR, False Discovery Rate; IPG, Immobilized pH Gradient; RMSD, Root-mean-square deviation; SVM, Support Vector MachineIsoelectric point; Support vector machine; Peptide descriptors
Identification and quantification of differentially expressed proteins in plasma of spinocerebellar ataxia type 12
Keywords: IPG، gradient of immobilized pH; SCA12, spinocerebellar ataxia type 12; 2D-DIGE, two-dimensional difference in-gel electrophoresis; ICARS, International Co-operative Ataxia Rating Scale; IPG, immobilized pH gradient; apo, apolipoprotein; TTR, transthyretin; Hpt, haptaglobin; Hx, hemopexi
Identification of novel serpin isoforms and serpin polymorphisms among Australian wheat cultivars
Keywords: IPG، gradient of immobilized pH; Serpin; Polymorphism; Australian wheat2-DE, two-dimensional gel electrophoresis; DTT, dithiothreitol; ESI, electrospray ionization; IEFfocussing, isoelectric; IPG, immobilized pH gradient; MS/MS, tandem mass spectrometry; PVDF, polyvinylidene difluoride;
Effects of arsenite stress on growth and proteome of Klebsiella pneumoniae
Keywords: IPG، gradient of immobilized pH; CHAPS, 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate; DTT, dithiothreitol; IEF, isoelectric focusing; IPG, immobilized pH gradient; MALDI-TOF-TOF, matrix assisted laser desorption ionization-time of flight-time of flightKlebsiella pneumoniae;
Postnatal growth velocity modulates alterations of proteins involved in metabolism and neuronal plasticity in neonatal hypothalamus in rats born with intrauterine growth restriction
Keywords: IPG، gradient of immobilized pH; IUGR, intrauterine growth retardation; PND, postnatal day; CC, control; RC, IUGR with rapid postnatal catch-up growth; RR, IUGR with slow postnatal growth; 2-DE, two-dimensional gel electrophoresis; IPG, immobilized pH gradient; SDS, sodium dodecyl sulpha
Fractionation and characterization of teff proteins
Keywords: IPG، gradient of immobilized pH; Teff; Sorghum; Prolamin; Electrophoresis2-D, two-dimensional; AL + GL, albumins + globulins; ATP, adenosine triphosphate; DTT, 1,4-dithiothreitol; IEF, isoelectric focusing; IPG, immobilized pH gradient; kDA, kilo Daltons; LMWNC, low molecular weight nitr
Proteomic analysis of responses of a new probiotic bacterium Lactobacillus casei Zhang to low acid stress
Keywords: IPG، gradient of immobilized pH; 2-DE, two dimensional electrophoresis; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; IPG, immobilized pH gradient; MS, mass spectrometry; MRS, de Man, Rogosa and Sharpe; DTT, dithiothreitol; COGs, clusters of orthologous groups; ATR
Altered sialylation on the cell-surface proteins of dexamethasone-treated human macrophages contributes to augmented uptake of apoptotic neutrophils
Keywords: IPG، gradient of immobilized pH; MDMΦ, monocyte-derived macrophage; Dex, dexamethasone; MERTK, Mer tyrosine kinase; CBB, Coomassie Brillant Blue; IPG, immobilized pH gradient; M-CSF, macrophage colony stimulating factor; SNA, Sambucus nigra agglutinin; CHAPS, 3-[(3-cholamidopropyl)-dimet
Polymorphism and pedigree analysis of β-amylase isozymes in Australian wheat
Keywords: IPG، gradient of immobilized pH; Wheat; β-Amylase; Polymorphism; Pedigree analysis2-DE, two-dimensional gel electrophoresis; DTT, dithiothreitol; ESI, electrospray ionization; IEF, isoelectric focussing; IPG, immobilized pH gradient; MAb, monoclonal antibody; MS/MS, tandem mass spectrome
Alterations in protein and gene expression within the barrel cortices of ZnT3 knockout mice: Experience-independent and dependent changes
Keywords: IPG، gradient of immobilized pH; CaM, calcium/calmodulin; CaMKII, calcium/calmodulin-dependent protein kinase type II; IPG, immobilized pH gradient; IEF, isoelectric focusing; KO, knockout; LS–MS/MS, liquid chromatography tandem mass spectrometry; MIQE, minimum information for publicatio
Comparative protein profiles: Potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces)
Keywords: IPG، gradient of immobilized pH; MALDI-TOF/MS, matrix-associated laser desorption/ionization time-of-flight/mass spectrometry; 2DE, two-dimensional polyacrylamide gel electrophoresis; IPG, immobilized pH gradient; MeCN, acetonitrile; pI, isoelectric point; MM, molecular mass; PMF, peptid
Identification and analysis of differentially expressed proteins during cotyledon embryo stage in longan
Keywords: IPG، gradient of immobilized pH; SE, somatic embryogenesis; DAA, days after anthesis; 2-DE, two-dimensional gel electrophoresis; MALDI-TOF-MS/MS, matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry; IEF, isoelectric focusing; IPG, immobilized pH gradient;
The nuclear matrix shell proteome of human epidermis
Keywords: IPG، gradient of immobilized pH; 2DGE, two-dimensional gel electrophoresis; FT-ICR, Fourier transform-ion cyclotron resonance mass spectrometer; HBSS, Hanks balanced salt solution; IEF, isoelectric focusing; IPG, immobilized pH gradient; LC, liquid chromatography; LTQ-FT, linear ion trap
Metabolic molecular indicators of chronic stress in gilthead seabream (Sparus aurata) using comparative proteomics
Keywords: IPG، gradient of immobilized pH; 2-DE, Two-dimensional gel electrophoresis; CHAPS, 3-[(3-cholamidopropyl) dimethylamonio]-1-propanesulphonate; CID, collision-induced dissociation; Da, dalton (molecular mass); DTT, Dithiothreitol; IPG, immobilized pH gradient; LC–MS/MS, liquid chromatogra
Protein disulfide isomerase-P5, down-regulated in the final stage of boar epididymal sperm maturation, catalyzes disulfide formation to inhibit protein function in oxidative refolding of reduced denatured lysozyme
Keywords: IPG، gradient of immobilized pH; PDI-P5, protein disulfide isomerase P5; PDIA3, protein disulfide isomerase A3; PBS, phosphate-buffered saline; DTT, dithiothreitol; IPG, immobilized pH gradient; IEF, isoelectric focusing; BPB, Bromophenol Blue; 2-DE, two-dimensional gel electrophoresis;
Echinococcus multilocularis: Proteomic analysis of the protoscoleces by two-dimensional electrophoresis and mass spectrometry
Keywords: IPG، gradient of immobilized pH; 2-DE, two-dimensional electrophoresis; CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]propanesulfonic acid; DTT, dithiothreitol; IEF, isoelectric focusing; IPG, immobilized pH gradient; MALDI-TOF MS, matrix-assisted laser desorption and ionization-time of f
Altered expression of HSPA5, HSPA8 and PARK7 in spinocerebellar ataxia type 17 identified by 2-dimensional fluorescence difference in gel electrophoresis
Keywords: IPG، gradient of immobilized pH; 2D-DIGE, two-dimensional difference in gel electrophoresis; HSPA5, heat shock 70 kDa protein 5; HSPA8, heat shock 70 kDa protein 8; IPG, immobilized pH gradient; PARK7, Parkinson disease 7; PMF, peptide mass fingerprinting; polyQ, polyglutamine; SCA17, sp
Transcriptomic and proteomic approach to studying SNX-2112-induced K562 cells apoptosis and anti-leukemia activity in K562-NOD/SCID mice
Keywords: IPG، gradient of immobilized pH; 17-AAG, 17-(allylamino)-17-demethoxygeldanmycin; DMSO, dimethylsulfoxide; CML, chronic myeloid leukemia; FBS, fetal bovine serum; IEF, isoelectric focusing; IPG, immobilized pH gradient; PRDX5, peroxiredoxin V; DLD, dihydrolipoamide dehydrogenase; ECH1, e
Development of the female gametophyte in the sterile ecotype of the bolting Allium sativum L.
Keywords: IPG، gradient of immobilized pH; DTT, dithiothreitol; IEF, isoelectrofocusing; IPG, immobilized pH gradient; PAGE, polyacrylamide gel electrophoresis; SDS, sodium dodecyl sulfateOvule abortion; Megasporogenesis; Embryo sac; Protein; Garlic
Discovery of lipoprotein lipase pI isoforms and contributions to their characterization
Keywords: IPG، gradient of immobilized pH; 2DE, two-dimensional electrophoresis; AP, alkaline phosphatase; CapLC-MS/MS, capillary liquid chromatography tandem mass spectrometry; IEF, isoelectric focusing; IPG, immobilized pH gradient; LPL, lipoprotein lipase; MALDI-TOF, matrix-assisted laser desor
The three classes of wheat xylanase-inhibiting proteins accumulate in an analogous way during wheat ear development and germination
Keywords: IPG، gradient of immobilized pH; DPA, days post-anthesis; DAS, days after steeping; GH, glycoside hydrolase; IPG, immobilized pH gradient; PAbs, polyclonal antibodies; PBS, phosphate-buffered saline; PGIP, polygalacturonase inhibitor; TAXI, Triticum aestivum xylanase inhibitor; TLXI, tha
Opposite effects of PDGF-BB and prostaglandin E1 on cell-motility related processes are paralleled by modifications of distinct actin-binding proteins
Keywords: IPG، gradient of immobilized pH; 2D, two dimensional; db-cAMP, di-butyryl cAMP; DIGE, difference in gel electrophoresis; ERK, extracellular signal-regulated kinase; ERM, ezrin, radixin, and moesin; IEF, isoelectric focusing; IPG, immobilized pH gradient; MALDI, matrix assisted laser deso
Proteomic analysis of the effect of bile salts on the intestinal and probiotic bacterium Lactobacillus reuteri
Keywords: IPG، gradient of immobilized pH; CHAPS, 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate; DTT, dithiothreitol; IEF, isoelectric focusing; IPG, immobilized pH gradient; MALDI–TOF, matrix-assisted laser desorption/ionization–time-of-flight; LAB, lactic acid bacteriaLactobacillus
Proteomics and mass spectrometric studies reveal planktonic growth of Mycobacterium smegmatis in biofilm cultures in the absence of rpoZ
Keywords: IPG، gradient of immobilized pH; IPG, Immobilized pH gradient; MALDI-TOF, Matrix-assisted laser desorption ionization time of flightMycobacteria; Biofilm; Proteomics; Mycolates; Glycopeptidolipids
Molecular cloning, expression and immunological characterisation of Pas n 1, the major allergen of Bahia grass Paspalum notatum pollen
Keywords: IPG، gradient of immobilized pH; BaGP, Bahia grass pollen; CBB, Coomassie Brilliant Blue R250; ELISA, enzyme linked immunosorbent assay; IPG, immobilized pH gradient; MP/PBST, milk powder in phosphate buffered saline with Tween 20; RACE, rapid amplification of cDNA ends; SDS-PAGE, sodium
Entamoeba histolytica: Response of the parasite to metronidazole challenge on the levels of mRNA and protein expression
Keywords: IPG، gradient of immobilized pH; Entamoeba histolytica; Metronidazole; Microarray; Proteomics; 2DE, two-dimensional electrophoresis; EC number, enzyme commission number; DTT, dithiothreitol; IPG, immobilized pH gradient; PFOR, pyruvate ferredoxin oxidoreductase; qRT-PCR, quantitative rea
Analysis of grape berry cell wall proteome: A comparative evaluation of extraction methods
Keywords: IPG، gradient of immobilized pH; Cell wall; LC-ESI-MS/MS; Proteomics; Two-dimensional gel electrophoresis; Vitis viniferaCWEf, cell wall-enriched fraction; CYf, cytosolic fraction; 2-DE, two-dimensional gel electrophoresis; IEF, isoelectric focusing; IPG, immobilized pH gradient; LC-ESI-
Characterization of proliferating cell nuclear antigen (PCNA) isoforms in normal and cancer cells: There is no cancer-associated form of PCNA
Keywords: IPG، gradient of immobilized pH; IEF, isoelectrofocusing; IPG, immobilized pH gradient; 2D-PAGE, two-dimensional polyacrylamide gel electrophoresis; CHAPS, 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate; ACN, acetonitrile; ABC, ammonium bicarbonate; CHCA, α-cyano-4-hydroxycin
Isoelectric point determination of cardiac troponin I forms present in plasma from patients with myocardial infarction
Keywords: IPG، gradient of immobilized pH; AMI, acute myocardial infarction; cTnC, cardiac troponin C; cTnI, cardiac troponin I; cTnT, cardiac troponin T; DTT, dithiothreitol; IPG, immobilized pH gradient; MW, molecular weight; PKA, protein kinase A; PVDF, polyvinylidene fluoride; SELDI-TOF MS, Su
Screening and secretomic analysis of enthomopatogenic Beauveria bassiana isolates in response to cowpea weevil (Callosobruchus maculatus) exoskeleton
Keywords: IPG، gradient of immobilized pH; MM, Minimal Medium; MMC, Minimal Medium + 0.5% C. maculatus exoskeleton; PDA, potato dextrose agar; 3.5 DNS, 3.5 dinitrosalicylic acid; IPG, immobilized pH gradient; DTT, dithiothreitol; SDS-PAGE, sodium dodecylsulfate-polyacrylamide gel electrophoresisCh
Acrolein induces selective protein carbonylation in synaptosomes
Keywords: IPG، gradient of immobilized pH; AD, Alzheimer’s disease; DNP, 2,4-dinitrophenyl hydrazone; EF-Tumt, mitochondrial Tu translation elongation factor; GSH, reduced glutathione; GS-propionaldehyde, glutathionylpropionaldehyde; IPG, immobilized pH gradient; MF, microfilament; TFA, trifluoroa
Extraction of wheat endosperm proteins for proteome analysis
Keywords: IPG، gradient of immobilized pH; 2-DE, two-dimensional polyacrylamide gel electrophoresis; IEF, isoelectric focusing; IPG, immobilized pH gradient; PAGE, polyacrylamide gel electrophoresis2-DE; Albumins; Amyloplasts; Endosperm; Gliadins; Globulins; Glutenins; Proteome; Wheat
Proteomic and genomic characterization of Kunitz trypsin inhibitors in wild and cultivated soybean genotypes
Keywords: IPG، gradient of immobilized pH; IEF, isoelectric focusing; IPG, immobilized pH gradient; KTI, Kunitz trypsin inhibitors; MALDI-TOF-MS, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; MOWSE, molecular weight search; pI, isoelectric points; 2D-PAGE, two-dimen
Proteomic analysis of whey and casein proteins in early milk from the marsupial Trichosurus vulpecula, the common brushtail possum
Keywords: IPG، gradient of immobilized pH; 2-D, two dimensional; conA, concanavalin A; ELP, early lactation protein; IPG, immobilized pH gradient; LLP, late lactation protein; MS/MS, tandem mass spectrometry; NCBInr, National Center for Biotechnology Information non-redundant protein sequence data
Two-dimensional electrophoresis analyses of atopic dermatitis and the chances to detect new candidate proteins by the variations in immobilized pH gradient strips
Keywords: IPG، gradient of immobilized pH; 2D-PAGE, two-dimensional electrophoresis; IPG, immobilized pH gradient; MALDI-TOF, matrix-assisted laser desorption/ionization time-of-flight; AD, atopic dermatitis; ADe, extrinsic type of AD; ADi, intrinsic type of AD2D-PAGE; IPG strip variation; Atopic
Changes in self-reactive IgG antibody repertoire after treatment of experimental autoimmune encephalomyelitis with anti-allergic drugs
Keywords: IPG، gradient of immobilized pH; CCB, Coomassie Colloïdal Blue; CFA/BPT, complete Freund adjuvant/Bordella pertussis toxin; HRP, horse radish peroxidase; IPG, Immobilized pH gradient; LDA, Linear Discriminant Analysis; MALDI-TOF, Matrix-Assisted Laser Desorption Ionization-Time Of Flight
Changes in disulfide bond content of proteins in a yeast strain lacking major sources of NADPH
Keywords: IPG، gradient of immobilized pH; CID, collision-induced dissociation; DTT, dithiothreitol; 6-IAF, 6-iodoacetamidofluorescein; IPG, immobilized pH gradient; MALDI-TOF/MS, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; TCA, trichloroacetic acidNADPH; Isocitra
The saliva proteome of the blood-feeding insect Triatoma infestans is rich in platelet-aggregation inhibitors
Keywords: IPG، gradient of immobilized pH; IEF, isoelectric focusing; IPG, immobilized pH gradient; 2-DE, two-dimensional gel electrophoresis; MS BLAST, mass spectrometry driven BLAST; TiAPY, Triatoma infestans apyrase; DTT, dithiothreitolTriatoma infestans; Saliva proteomic; Chagas disease; Tande
The nuclear proteome and DNA-binding fraction of human Raji lymphoma cells
Keywords: IPG، gradient of immobilized pH; 2-DE, two-dimensional gel electrophoresis; 2-DLC, two-dimensional liquid chromatography; IPG, immobilized pH gradient; MALDI-TOF, matrix-assisted laser desorption-ionization time-of-flight; MS, mass spectrometry; MS-MS, tandem mass spectrometry; pI, iso-e
Screening of entomopathogenic Metarhizium anisopliae isolates and proteomic analysis of secretion synthesized in response to cowpea weevil (Callosobruchus maculatus) exoskeleton
Keywords: IPG، gradient of immobilized pH; MM, minimal medium; MMC, minimal medium + 0.5% C. maculatus exoskeleton; PDA, potato dextrose agar; 3.5 DNS, 3.5 dinitrosalicylic acid; IPG, immobilized pH gradient; DTT, dithiothreitol; SDS–PAGE, sodium dodecylsulfate–polyacrylamide gel electrophoresisCh
Lipopolysaccharide-responsive phosphoproteins in Nicotiana tabacum cells
Keywords: IPG، gradient of immobilized pH; Innate immunity; Lipopolysaccharides; Phosphoproteins; Plant defense; Proteomics; Signal transduction2D, two dimensional; ESI, electrospray ionization; Flg22, flagellin-22 peptide; IEF, isoelectric focusing; IPG, immobilized pH gradient; LC, liquid chroma