کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2014948 1541959 2013 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Proteomic analysis of “Moncada” mandarin leaves with contrasting fruit load
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Proteomic analysis of “Moncada” mandarin leaves with contrasting fruit load
چکیده انگلیسی

A proteomic approach was used to know more about the molecular mechanism related to Citrus alternate bearing. To this end, we researched protein expression differences between on-crop and off-crop “Moncada” [Clementine ‘Oroval’ (Citrus clementina Hort ex Tanaka) x ‘Kara’ mandarin (Citrus unshiu Marc. x Citrus nobilis Lou.)] mandarin leaves. This variety usually shows a remarkable behaviour in alternate production. Samples were collected in the period during which the fruit affect flowering induction. From 2D DIGE gel, 110 spots were isolated: 43 showed increased expression in the off-crop samples compared to on-crop samples, while 67 showed increased expression in the on-crop samples against off-crop samples. These spots were identified by MALDI-MS or LC-MS–MS. According to the up-expressed proteins in off-crop leaves such as proteins related to nutrient reservoir activity or to the pentose phosphate pathway, the primary metabolism was more active in off-crop trees than in on-crop trees. In contrast, the proteins up-expressed in on-crop samples such as catalase were related to the oxidoreductase activity and, therefore, the redox state seemed different for off-crop and for on-crop leaves. Other proteins with unknown functions were isolated, which could be also related to the alternate bearing and to the flowering induction.


► A proteomic study was did to know molecular mechanisms of Citrus alternate bearing.
► The variety studied was “Moncada” mandarin, in the period of flowering induction.
► We researched protein expression differences between on-crop and off-crop leaves.
► The primary metabolism seemed more active in off-crop trees than in on-crop trees.
► Proteins up-expressed in on-crop leaves were more related to oxidoreductase activity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 62, January 2013, Pages 95–106
نویسندگان
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