کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4372106 1617030 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Entamoeba histolytica: Response of the parasite to metronidazole challenge on the levels of mRNA and protein expression
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Entamoeba histolytica: Response of the parasite to metronidazole challenge on the levels of mRNA and protein expression
چکیده انگلیسی

Entamoeba histolytica remains a cause of significant morbidity and mortality in many countries. Although metronidazole has been used for the treatment of amoebiasis for several decades, little is known on how the amoebae react to this challenge on the levels of mRNA and protein expression. In this study, we examined their response using a focused microarray, quantitative RT-PCR, and two-dimensional gel electrophoresis (2DE). The amoebae modestly increased the levels of mRNA coding for superoxide dismutase, peroxiredoxin, ferredoxin, thioredoxin reductase, and the galactose/N-acetylgalactosamine specific lectin light and heavy chains. The mRNAs encoding actin and the 70 kDa and 101 kDa heat shock proteins were decreased. All the changes occured within 1 h of exposition, with very little further changes. In addition, the proteome revealed only very few changes. Taken together, E. histolytica appears to make only modest mRNA and protein expression changes when confronted with an unknown chemical stress.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 120, Issue 4, December 2008, Pages 403–410
نویسندگان
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