کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2051040 1074189 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Stereoisomeric bio-inversion key to biosynthesis of firefly d-luciferin
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Stereoisomeric bio-inversion key to biosynthesis of firefly d-luciferin
چکیده انگلیسی

The chirality of the luciferin substrate is critical to the luciferin–luciferase reaction producing bioluminescence. In firefly, the biosynthetic pathway of d-luciferin is still unclear, although it can be synthesized in vitro from d-cysteine. Here, we show that the firefly produces both d- and l-luciferin, and that the amount of active d-luciferin increases gradually with maturation stage. Studies of firefly body extracts indicate the possible conversion of l-cysteine via l-luciferin into d-luciferin, suggesting that the biosynthesis is enzymatically regulated by stereoisomeric bio-inversion of l-luciferin. We conclude that the selection of chirality in living organisms is not as rigid as previously thought.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 580, Issue 22, 2 October 2006, Pages 5283–5287
نویسندگان
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