کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2051358 1074197 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel strong competitive inhibitor of complex I
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
A novel strong competitive inhibitor of complex I
چکیده انگلیسی

Alkaline incubation of NADH results in the formation of a very potent inhibitor of complex I (NADH:ubiquinone oxidoreductase). Mass spectroscopy (molecular mass equal to 696) and absorption spectroscopy suggest that the inhibitor is derived from attachment of two oxygen atoms to the nicotinamide moiety of NADH. The inhibitor is competitive with respect to NADH with a Ki of about 10−8 M. The inhibitor efficiently suppresses NADH-oxidase, NADH-artificial acceptor reductase, and NADH-quinone reductase reactions catalyzed by submitochondrial particles, as well as the reactions catalyzed by either isolated complex I or the three subunit flavoprotein fragment of complex I.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 579, Issue 21, 29 August 2005, Pages 4861–4866
نویسندگان
, , ,