کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2051375 | 1074198 | 2007 | 6 صفحه PDF | دانلود رایگان |
The potential role of cysteine cathepsins, especially cathepsin B, in Fas/CD95-induced apoptosis was investigated using wild-type and cathepsin B-deficient primary skin fibroblasts. Apoptosis was induced with an anti-Fas/CD95 antibody in the presence of cycloheximide and no difference was observed between the two genotypes. First cells with damaged mitochondria were observed ∼3 h post apoptosis induction and their number was significantly increased after 11 h. In contrast, cells with damaged lysosomes were only seen after 15 h with no difference between the two genotypes. Moreover, Bid cleavage was found to be diminished in cathepsin B-deficient cells. These results suggest that cysteine cathepsins have no active role in Fas/CD95 apoptosis.
Journal: FEBS Letters - Volume 581, Issue 27, 13 November 2007, Pages 5185–5190