کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2051551 1074203 2007 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fatty acid chain-elongation in perfused rat heart: Synthesis of stearoylcarnitine from perfused palmitate
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Fatty acid chain-elongation in perfused rat heart: Synthesis of stearoylcarnitine from perfused palmitate
چکیده انگلیسی

Rat hearts perfused for up to 60 min in the working mode with palmitate, but not with glucose, resulted in substantial formation of palmitoylcarnitine and stearoylcarnitine. To test whether lipolysis of endogenous lipids was responsible for the increased stearoylcarnitine content or whether some of the perfused palmitate underwent chain elongation, hearts were perfused with hexadecanoic-16,16,16-d3 acid (M+3). The pentafluorophenacyl ester of deuterium labeled stearoylcarnitine had an M+3 (639.4 m/z) compared to the unlabeled M+0 (636.3 m/z) consistent with a direct chain elongation of the perfused palmitate. Furthermore, the near equal isotope enrichment of palmitoyl- (90.2 ± 5.8%) and stearoylcarnitine (78.0 ± 7.1%) suggest that both palmitoyl- and stearoyl-CoA have ready access to mitochondrial carnitine palmitoyltransferase and that most of the stearoylcarnitine is derived from the perfused palmitate.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 581, Issue 23, 18 September 2007, Pages 4491–4494
نویسندگان
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