کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2051697 1074207 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Heme binding properties of heterologously expressed spinach cytochrome b6: Implications for transmembrane b-type cytochrome formation
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Heme binding properties of heterologously expressed spinach cytochrome b6: Implications for transmembrane b-type cytochrome formation
چکیده انگلیسی

In vivo and in vitro requirements for the formation of cytochrome b6 were examined to analyze the mechanisms of transmembrane b-type cytochrome formation. After heterologous expression of spinach cytochrome b6, formation of the holo-cytochrome was observed within the E. coli inner membrane. The transmembrane orientation of cytochrome b6 appeared not to be critical for heme binding and holo-cytochrome formation. Furthermore, in vitro reconstitution of cytochrome b6 was possible under oxidizing as well as under reducing conditions. Taken together these observations strongly indicate that transmembrane b-type cytochromes can spontaneously assemble in vitro as well as in a membrane.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 581, Issue 14, 12 June 2007, Pages 2647–2651
نویسندگان
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