کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2051698 | 1074207 | 2007 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Synthesis of globopentaose using a novel β1,3-galactosyltransferase activity of the Haemophilus influenzae β1,3-N-acetylgalactosaminyltransferase LgtD Synthesis of globopentaose using a novel β1,3-galactosyltransferase activity of the Haemophilus influenzae β1,3-N-acetylgalactosaminyltransferase LgtD](/preview/png/2051698.png)
We have previously described a bacterial system for the conversion of globotriaose (Gb3) into globotetraose (Gb4) by a metabolically engineered Escherichia coli strain expressing the Haemophilus influenzae lgtD gene encoding β1,3-N-acetylgalactosaminyltransferase [Antoine, T., Bosso, C., Heyraud, A. Samain, E. (2005) Large scale in vivo synthesis of globotriose and globotetraose by high cell density culture of metabolically engineered Escherichia coli. Biochimie 87, 197–203]. Here, we found that LgtD has an additional β1,3-galactosyltransferase activity which allows our bacterial system to be extended to the synthesis of the carbohydrate portion of globopentaosylceramide (Galβ-3GalNAcβ-3Galα-4Galβ-4Glc) which reacts with the monoclonal antibody defining the stage-specific embryonic antigen-3. In vitro assays confirmed that LgtD had both β1,3-GalT and β1,3-GalNAcT activities and showed that differences in the affinity for Gb3 and Gb4 explain the specific and exclusive formation of globopentaose.
Journal: FEBS Letters - Volume 581, Issue 14, 12 June 2007, Pages 2652–2656