کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2052354 1074228 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nek7 kinase is enriched at the centrosome, and is required for proper spindle assembly and mitotic progression
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Nek7 kinase is enriched at the centrosome, and is required for proper spindle assembly and mitotic progression
چکیده انگلیسی

Members of the NIMA-related kinases (NRK) family are recently emerging as central regulators of various aspects of the cell cycle. However, the cellular roles of the mammalian NRK, Nek7, remain obscure. We show here that the endogenous Nek7 protein is enriched at the centrosome in a microtubule-independent manner. Overexpression of wt or kinase-defective Nek7 resulted in cells of rounder appearance, and higher proportions of multinuclear and apoptotic cells. Down-regulation of Nek7 using a small interfering RNA approach resulted in a significant increase in mitotic cells presenting multipolar spindle phenotype. These results suggest a role for Nek7 in regulating proper spindle assembly and mitotic progression.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 580, Issue 27, 27 November 2006, Pages 6489–6495
نویسندگان
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