کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2052484 1074231 2006 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells: Intracellular concentration decrease in NAD and increase in cyclic ADP-ribose
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells: Intracellular concentration decrease in NAD and increase in cyclic ADP-ribose
چکیده انگلیسی

ADP-ribosyl cyclase activity in the crude membrane fraction of neuroblastoma × glioma NGPM1-27 hybrid cells was measured by monitoring [3H] cyclic ADP-ribose (cADPR) formation from [3H] NAD+. Bradykinin (BK) at 100 nM increased ADP-ribosyl cyclase activity by about 2.5-fold. Application of 300 nM BK to living NGPM1-27 cells decreased NAD+ to 78% of the prestimulation level at 30 s. In contrast, intracellular cADPR concentrations were increased by 2–3-fold during the period from 30 to 120 s after the same treatment. Our results suggest that cADPR is one of the second messengers downstream of B2 BK receptors.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 580, Issue 20, 4 September 2006, Pages 4857–4860
نویسندگان
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