کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2052566 | 1074233 | 2005 | 6 صفحه PDF | دانلود رایگان |
The rat catalase gene carries a TATA-less promoter and its transcriptional mechanism is interesting because of downregulation in liver injury. We characterized the core element in the promoter and found that C/EBP-β binding downstream of the transcription initiation site plays a crucial role for transcription. The multiple complexes binding to the promoter were composed of homodimers and heterodimers of C/EBP-β isoforms. Transduction of the C/EBP-β gene showed complete reconstitution of multiple binding complexes in HeLa cells, similar to normal liver. Furthermore, C/EBP-β was observed to bind to the endogenous catalase promoter. These data suggest that multiple complex formation of C/EBP-β regulates transcription in the TATA-less catalase promoter.
Journal: FEBS Letters - Volume 579, Issue 25, 24 October 2005, Pages 5785–5790