کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2052650 | 1074235 | 2006 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transient suppression of PPARγ directed ES cells into an osteoblastic lineage
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Transient suppression of PPARγ directed ES cells into an osteoblastic lineage Transient suppression of PPARγ directed ES cells into an osteoblastic lineage](/preview/png/2052650.png)
چکیده انگلیسی
Osteoblasts and adipocytes are believed to share a common progenitor. Peroxisome proliferator-activated receptor γ (PPARγ) plays a key role in the switching of these two cell lineages. Here, we demonstrated the differentiation of ES cells into an osteoblastic lineage using siRNA against PPARγ without the addition of any osteogenic factors. We found that PPARγ-siRNA downregulated the expression of aP2 mRNA and lipid accumulation, whereas it upregulated the expression of osteocalcin and calcium deposition. These results suggested that ES cells were directed into an osteoblastic lineage. Therefore, transient suppression using PPARγ-siRNA may be a novel tool to induce differentiation of ES cells into osteoblasts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 580, Issue 17, 24 July 2006, Pages 4121–4125
Journal: FEBS Letters - Volume 580, Issue 17, 24 July 2006, Pages 4121–4125
نویسندگان
Akihiro Yamashita, Tatsuyuki Takada, Ken-ichi Nemoto, Gaku Yamamoto, Ryuzo Torii,