کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2052802 1074240 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Substrate specificity and preference of Δ6-desaturase of Mucor rouxii
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Substrate specificity and preference of Δ6-desaturase of Mucor rouxii
چکیده انگلیسی

The Δ6-fatty acid desaturase is a key enzyme in the synthesis of an important fatty acid, γ-linolenic acid. We have characterized, by heterologous expression in Saccharomyces cerevisiae, substrate specificity and preference of Δ6-desaturase of Mucor rouxii. Fatty acid supplementation was carried out based on the predicted enzyme topology, fatty acid phenotype and the corresponding metabolic pathway in M. rouxii. The enzyme has a broad substrate specificity as based on C15–C18. The result also supported classification of the M. rouxii Δ6-desaturase into a front-end desaturase. Interestingly, a relatively rare activity based on odd acyl chains and not described previously in other eukaryotic Δ6-desaturases was also observed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 579, Issue 12, 9 May 2005, Pages 2744–2748
نویسندگان
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