کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2053003 1074251 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Differential gene expression in egg cells and zygotes suggests that the transcriptome is restructed before the first zygotic division in tobacco
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Differential gene expression in egg cells and zygotes suggests that the transcriptome is restructed before the first zygotic division in tobacco
چکیده انگلیسی

We applied suppression subtractive hybridization and mirror orientation selection to compare gene expression profiles of isolated Nicotiana tabacum cv SR1 zygotes and egg cells. Our results revealed that many differentially expressed genes in zygotes were transcribed de novo after fertilization. Some of these genes are critical to zygote polarity and pattern formation during early embryogenesis. This suggests that the transcriptome is restructed in zygote and that the maternal-to-zygotic transition happens before the first zygotic division, which is much earlier in higher plants than in animals. The expressed sequence tags used in this study provide a valuable resource for future research on fertilization and early embryogenesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 580, Issue 7, 20 March 2006, Pages 1747–1752
نویسندگان
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