کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2053063 | 1074254 | 2006 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: High phosphorylation of HBV core protein by two α-type CK2-activated cAMP-dependent protein kinases in vitro High phosphorylation of HBV core protein by two α-type CK2-activated cAMP-dependent protein kinases in vitro](/preview/png/2053063.png)
Two α-type CK2-activated PKAs (CK2-aPKAIα and CK2-aPKAIIα) were biochemically characterized in vitro using GST-HBV core fusion protein (GST-Hcore) and GST-Hcore157B as phosphate acceptors. It was found that (i), in the absence of cAMP, these two CK2-aPKAs phosphorylated both Ser-170 and Ser-178 on GST-Hcore and Hcore157B; (ii) this phosphorylation was approx. 4-fold higher than their phosphorylation by cAMP-activated PKAs; and (iii) suramin effectively inhibited the phosphorylation of Hcore157B by CK2-aPKAIIα through its direct binding to Hcore157B in vitro. These results suggest that high phosphorylation of HBV-CP by two CK2-aPKAs, in the absence of cAMP, may be involved in the pregenomic RNA (pgRNA) encapsidation and DNA-replication in HBV-infected cells.
Journal: FEBS Letters - Volume 580, Issue 3, 6 February 2006, Pages 894–899