کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2054177 1543658 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Leaf and powdery mildew colonization by glycolipid-producing Pseudozyma species
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
Leaf and powdery mildew colonization by glycolipid-producing Pseudozyma species
چکیده انگلیسی
In this work, we followed the development and compared the biocontrol potential of four phylogenetically related species of Pseudozyma (P. antarctica, P. flocculosa, P. fusiformata and P. rugulosa) all known to release glycolipids with antifungal activity in vitro. To this end, we developed GFP transformants and specific primers for each species and conducted in situ observations and quantification of their population sizes over time on both healthy and powdery mildew-infected leaves. Although all species shared many similar features, only P. flocculosa antagonized powdery mildew colonies and grew specifically in their presence. Population quantification by qRT-PCR following inundative applications showed a drastic decline in population for all species, including P. flocculosa on healthy leaves, but a sharp and steady increase of the latter over 72 h on infected leaves. These results suggest that production of glycolipids, and more specifically flocculosin or ustilagic acid, is not the sole factor dictating biocontrol activity among Pseudozyma spp.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Ecology - Volume 1, Issues 2–3, May–August 2008, Pages 69-77
نویسندگان
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