کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2056144 1075804 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of nuclear localization signal in ASYMMETRIC LEAVES2-LIKE18/LATERAL ORGAN BOUNDARIES DOMAIN16 (ASL18/LBD16) from Arabidopsis
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
Identification of nuclear localization signal in ASYMMETRIC LEAVES2-LIKE18/LATERAL ORGAN BOUNDARIES DOMAIN16 (ASL18/LBD16) from Arabidopsis
چکیده انگلیسی

The ASYMMETRIC LEAVES2-LIKE/LATERAL ORGAN BOUNDARIES DOMAIN (ASL/LBD) gene family encodes proteins harboring a conserved plant-specific LOB domain. The LOB domain contains a four-Cys motif, a Gly–Ala–Ser (GAS) block, and a Leu-zipper-like coiled-coil motif. The ASL/LBD proteins are a unique class of transcription factors that play roles in lateral organ development of plants. Although the ASL/LBD proteins are localized in the nucleus, no consensus sequence for the nuclear localization of the ASL/LBD proteins could be found. In the present study, we determined the motifs responsible for the nuclear localization of ASL18/LBD16 by using protoplast transfection assays with a variety N- or C-terminal deletion polypeptide fragments and the polypeptides harboring changes in basic amino acids that are fused to enhanced green fluorescent protein. The results demonstrated that ASL18/LBD16 harbors two distinct domains comprising an atypical nuclear localization signal (NLS) with basic amino acid residues in the coiled-coil motif and a monopartite-like NLS in the C-terminal region for nuclear targeting.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Plant Physiology - Volume 169, Issue 12, 15 August 2012, Pages 1221–1226
نویسندگان
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