کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2056881 1075848 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ascorbate–glutathione cycle of mitochondria in osmoprimed soybean cotyledons in response to imbibitional chilling injury
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
Ascorbate–glutathione cycle of mitochondria in osmoprimed soybean cotyledons in response to imbibitional chilling injury
چکیده انگلیسی

Osmopriming treatment of chilling-sensitive soybean (Glycine max L. cv. Zhonghuang-22) seeds for 72 h at 25 °C with polyethylene glycol (PEG8000) solution at −1.5 MPa strongly improves chilling resistance. The aim of the present work was to investigate whether the beneficial effect of osmopriming is associated with restoration of the ascorbate–glutathione (ASC–GSH) cycle of mitochondria in soybean seeds. Compared with the control, both H2O2 and malondialdehyde (MDA) contents in mitochondria of osmoprimed seeds decreased after chilling treatment, and these changes were associated with increased activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR) and l-galactono-γ-lactone dehydrogenase (GLDH). However, the activity of dehydroascorbate reductase (DHAR) showed no obvious change during osmopriming treatment. Increased ASC and GSH contents accompanied prolonged osmopriming, and the reduced/oxidized ratios of ASC and GSH increased differently during osmopriming. These results indicate that osmopriming treatment enhances activity of the ASC–GSH cycle of mitochondria, which raises the chilling tolerance in soybean seeds and protects against H2O2 that is generated in mitochondria during imbibition at low temperature.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Plant Physiology - Volume 168, Issue 3, 15 February 2011, Pages 226–232
نویسندگان
, , , , ,