کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2057119 | 1075863 | 2010 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Application of a genome walking method for the study of the spinach Lhcb1 multigene family
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
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چکیده انگلیسی
We describe the application of a novel genome walking (GW) strategy for the one-shot identification of members of multigene families. The method was used to study the spinach Lhcb1 family (encoding the light harvesting complex protein Lhcb1), for which three cDNAs were known. Two additional genes and regulatory regions of the five members of the family were identified. For one of the newly detected genes, sequencing of full-length cDNA and analysis of regulatory regions by gel-shift are also reported.To our best knowledge, this is the first report on the use of a GW approach for the study of a multigene family.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Plant Physiology - Volume 167, Issue 2, 15 January 2010, Pages 138–143
Journal: Journal of Plant Physiology - Volume 167, Issue 2, 15 January 2010, Pages 138–143
نویسندگان
Claudia Leoni, Mariateresa Volpicella, Antonio Placido, Raffaele Gallerani, Luigi R. Ceci,