کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2057150 | 1075865 | 2010 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Involvement of nitric oxide in the inhibition of nitrogenase activity by nitrate in Lotus root nodules
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
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چکیده انگلیسی
Nitrogenase activity, as acetylene-reduction activity (ARA), in Lotus root nodules was clearly inhibited 27 h after the addition of nitrate. Nitric oxide (NO) production was detected at that time in nitrate-supplied root nodules using the NO-reactive fluorescent probe diaminofluorescein-2 diacetate. The involvement of NO production in the inhibition of nitrogenase activity by nitrate was investigated using the NO donor sodium nitroprusside (SNP) and the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO). SNP inhibited ARA at 1 mM, and c-PTIO suppressed the inhibition of ARA by nitrate. These results suggest that NO is involved in the inhibition of nitrogenase activity by nitrate in Lotus root nodules.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Plant Physiology - Volume 167, Issue 3, 15 February 2010, Pages 238–241
Journal: Journal of Plant Physiology - Volume 167, Issue 3, 15 February 2010, Pages 238–241
نویسندگان
Kazuhisa Kato, Koki Kanahama, Yoshinori Kanayama,