کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2058031 | 1076144 | 2007 | 7 صفحه PDF | دانلود رایگان |
SummarySalinity is a major abiotic stress factor limiting crop production. To generate salt-tolerant turf and forage, we had transformed tall fescue (Festuca arundinacea) with AtNHX1, a vacuolar Na+/H+ antiporter gene from Arabidopsis thaliana. In this paper, we report that overexpression of the AtNHX1 gene confers enhanced salt tolerance to the transformed tall fescue progenies. DNA gel blot analysis and reverse transcription (RT) polymerase chain reaction (PCR) were carried out to confirm the inheritance and expression of the AtNHX1 gene in transgenic T1 and T2 lines. These transgenic lines showed no phenotypic changes or yield reduction. Plants carrying the AtNHX1 gene were more resistant to a 200 mM NaCl solution than control plants. The roots of the transgenic lines had a higher sodium content than controls, due to an increased Na+/H+ antiporter activity in tonoplast vesicles. Our results suggest that this accumulation of sodium in vacuoles of root cells, mediated by vacuolar Na+/H+ antiporters, reduced the toxic effects of salinity to tall fescue and thus enhanced its salt tolerance.
Journal: Journal of Plant Physiology - Volume 164, Issue 10, 19 October 2007, Pages 1377–1383