Single tube tetraplex PCR based screening of a SNP at exon 14 region of bovine ITGB6 among different Zebu breeds

• A single tetra ARMS PCR based strategy to screen ITGB6 polymorphism was developed.
• TT genotypes are widely distributed among different Zebu breed.
• Identified SNP may leads to a missense variation.

The present study was aimed to screen genetic variation within a SNP (rs136500299) located at exon 14 region of bovine ITGB6 gene among different Zebu cattle breeds. The genotyping method describe in the present study is a tetraplex ARMS PCR, which offers extremely fast, economical, and simple detection tool. The distribution of the ITGB6 genotypes among the different breeds studied suggested that the populations were under Hardy–Weinberg equilibrium. Our findings revealed that TT genotypes are widely distributed among different Zebu cattle breeds, which can be associated with the resistance to FMD virus, as the Bos indicus are more resistant to FMD virus in comparison to Bos taurus.